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human lens epithelial b3 line hle cells  (ATCC)


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    ATCC human lens epithelial b3 line hle cells
    Human Lens Epithelial B3 Line Hle Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 647 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+lens+epithelial+b3+line+hle+cells/B-3/pmc12735461-449-0-10
    Average 97 stars, based on 647 article reviews
    human lens epithelial b3 line hle cells - by Bioz Stars, 2026-10
    97/100 stars

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    Cell Culture:

    Article Title: An Ongoing Search for Multitarget Ligands as Potential Agents for Diabetes Mellitus and Its Long-Term Complications: New Insights into (5-Arylidene-4-oxothiazolidin-3-yl)alkanoic Acid Derivatives
    Article Snippet: .. Human lens epithelial B3 line (HLE) cells were purchased from American Type Culture Collection (Rockville, MD, USA) and cultured at 37 °C in a humidified atmosphere, in the presence of 5% CO 2 , in an Eagle’s modified minimum essential medium (MEM) containing 5.5 mM D-glucose, supplemented with 20% ( v / v ) FBS, 50 mU/mL penicillin/streptomycin, 2 mM glutamine and 100 μg/mL hygromycin. ..

    Modification:

    Article Title: An Ongoing Search for Multitarget Ligands as Potential Agents for Diabetes Mellitus and Its Long-Term Complications: New Insights into (5-Arylidene-4-oxothiazolidin-3-yl)alkanoic Acid Derivatives
    Article Snippet: .. Human lens epithelial B3 line (HLE) cells were purchased from American Type Culture Collection (Rockville, MD, USA) and cultured at 37 °C in a humidified atmosphere, in the presence of 5% CO 2 , in an Eagle’s modified minimum essential medium (MEM) containing 5.5 mM D-glucose, supplemented with 20% ( v / v ) FBS, 50 mU/mL penicillin/streptomycin, 2 mM glutamine and 100 μg/mL hygromycin. ..



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    Fig. 2 The effects of apelin-13 on the cell viability of H2O2-treated <t>HLE-B3</t> cells. (a) HLE-B3 cells were incubated with various concentrations of apelin-13 (0.01 µM, 0.1 µM, 1 µM) for 24 h. (b) HLE-B3 cells were incubated with various concentrations of H2O2 (50 µM, 100 µM, 200 µM, 400 µM) for 24 h. (c) HLE- B3 cells were preincubated with apelin-13 (0.01 µM, 0.1 µM, 1 µM) for 24 h before being treated with H2O2 (200 µM) for 24 h. Cell viability was evaluated using CCK8 assay. Representative images of three replicated experiments were presented. The significance marked at the top of the columns refers to comparison with the control group. Data are shown as mean ± SD (n = 3)
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    Fig. 2 The effects of apelin-13 on the cell viability of H2O2-treated <t>HLE-B3</t> cells. (a) HLE-B3 cells were incubated with various concentrations of apelin-13 (0.01 µM, 0.1 µM, 1 µM) for 24 h. (b) HLE-B3 cells were incubated with various concentrations of H2O2 (50 µM, 100 µM, 200 µM, 400 µM) for 24 h. (c) HLE- B3 cells were preincubated with apelin-13 (0.01 µM, 0.1 µM, 1 µM) for 24 h before being treated with H2O2 (200 µM) for 24 h. Cell viability was evaluated using CCK8 assay. Representative images of three replicated experiments were presented. The significance marked at the top of the columns refers to comparison with the control group. Data are shown as mean ± SD (n = 3)
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    Fig. 2 The effects of apelin-13 on the cell viability of H2O2-treated HLE-B3 cells. (a) HLE-B3 cells were incubated with various concentrations of apelin-13 (0.01 µM, 0.1 µM, 1 µM) for 24 h. (b) HLE-B3 cells were incubated with various concentrations of H2O2 (50 µM, 100 µM, 200 µM, 400 µM) for 24 h. (c) HLE- B3 cells were preincubated with apelin-13 (0.01 µM, 0.1 µM, 1 µM) for 24 h before being treated with H2O2 (200 µM) for 24 h. Cell viability was evaluated using CCK8 assay. Representative images of three replicated experiments were presented. The significance marked at the top of the columns refers to comparison with the control group. Data are shown as mean ± SD (n = 3)

    Journal: BMC ophthalmology

    Article Title: Protective effect of apelin-13 in lens epithelial cells via inhibiting oxidative stress-induced apoptosis.

    doi: 10.1186/s12886-024-03746-6

    Figure Lengend Snippet: Fig. 2 The effects of apelin-13 on the cell viability of H2O2-treated HLE-B3 cells. (a) HLE-B3 cells were incubated with various concentrations of apelin-13 (0.01 µM, 0.1 µM, 1 µM) for 24 h. (b) HLE-B3 cells were incubated with various concentrations of H2O2 (50 µM, 100 µM, 200 µM, 400 µM) for 24 h. (c) HLE- B3 cells were preincubated with apelin-13 (0.01 µM, 0.1 µM, 1 µM) for 24 h before being treated with H2O2 (200 µM) for 24 h. Cell viability was evaluated using CCK8 assay. Representative images of three replicated experiments were presented. The significance marked at the top of the columns refers to comparison with the control group. Data are shown as mean ± SD (n = 3)

    Article Snippet: HLEC-B3 cells culture and drug treatment The human lens epithelial B3 (HLE-B3) cell line (CRL11421; ATCC, Manassas, Virginia, USA) was used in this experiment.

    Techniques: Incubation, CCK-8 Assay, Comparison, Control

    Fig. 3 Apelin-13 prevented H2O2-induced cellular viability in HLE-B3 cells. Viability of HLE-B3 cells as assessed using CCK8 assay. HLE-B3 cells were treated with ML221 (10 µmol) for 1 h, preincubated with apelin-13 (0.1 µM) for 24 h, and subsequently treated with H2O2 (200 µM) for 24 h in this experiment. Representative images of three replicated experiments were presented. The significance marked at the top of the columns refers to comparison with the control group. Data are shown as mean ± SD (n = 3)

    Journal: BMC ophthalmology

    Article Title: Protective effect of apelin-13 in lens epithelial cells via inhibiting oxidative stress-induced apoptosis.

    doi: 10.1186/s12886-024-03746-6

    Figure Lengend Snippet: Fig. 3 Apelin-13 prevented H2O2-induced cellular viability in HLE-B3 cells. Viability of HLE-B3 cells as assessed using CCK8 assay. HLE-B3 cells were treated with ML221 (10 µmol) for 1 h, preincubated with apelin-13 (0.1 µM) for 24 h, and subsequently treated with H2O2 (200 µM) for 24 h in this experiment. Representative images of three replicated experiments were presented. The significance marked at the top of the columns refers to comparison with the control group. Data are shown as mean ± SD (n = 3)

    Article Snippet: HLEC-B3 cells culture and drug treatment The human lens epithelial B3 (HLE-B3) cell line (CRL11421; ATCC, Manassas, Virginia, USA) was used in this experiment.

    Techniques: CCK-8 Assay, Comparison, Control

    Fig. 4 Apelin-13 prevented H2O2-induced cellular apoptosis in HLE-B3 cells. Based on the experimental statistics, apelin-13 substantially decreased the apoptosis rate of HLE-B3 cells through APJ. Representative images of three replicated experiments were presented. The significance marked at the top of the columns refers to comparison with the control group. Data are shown as mean ± SD (n = 3)

    Journal: BMC ophthalmology

    Article Title: Protective effect of apelin-13 in lens epithelial cells via inhibiting oxidative stress-induced apoptosis.

    doi: 10.1186/s12886-024-03746-6

    Figure Lengend Snippet: Fig. 4 Apelin-13 prevented H2O2-induced cellular apoptosis in HLE-B3 cells. Based on the experimental statistics, apelin-13 substantially decreased the apoptosis rate of HLE-B3 cells through APJ. Representative images of three replicated experiments were presented. The significance marked at the top of the columns refers to comparison with the control group. Data are shown as mean ± SD (n = 3)

    Article Snippet: HLEC-B3 cells culture and drug treatment The human lens epithelial B3 (HLE-B3) cell line (CRL11421; ATCC, Manassas, Virginia, USA) was used in this experiment.

    Techniques: Comparison, Control

    Fig. 5 Effect of apelin‑13 on mitochondrial apoptotic pathway in H2O2-induced HLE-B3 cells via APJ. (a, d) The fluorescence microscopy findings (×100) on mitochondrial membrane potential (MMP) in each group obtained using the fluorescence probe JC-1. (b, c) The expression of mitochondrial apop totic pathway-related proteins (Bax, Caspase-3, and Bcl-2) detected by western blotting. Representative images of three replicated experiments were presented. The significance marked at the top of the columns refers to comparison with the control group. Data are shown as mean ± SD (n = 3). The full-length blots/gels are presented in Supplementary Figs. 8–11

    Journal: BMC ophthalmology

    Article Title: Protective effect of apelin-13 in lens epithelial cells via inhibiting oxidative stress-induced apoptosis.

    doi: 10.1186/s12886-024-03746-6

    Figure Lengend Snippet: Fig. 5 Effect of apelin‑13 on mitochondrial apoptotic pathway in H2O2-induced HLE-B3 cells via APJ. (a, d) The fluorescence microscopy findings (×100) on mitochondrial membrane potential (MMP) in each group obtained using the fluorescence probe JC-1. (b, c) The expression of mitochondrial apop totic pathway-related proteins (Bax, Caspase-3, and Bcl-2) detected by western blotting. Representative images of three replicated experiments were presented. The significance marked at the top of the columns refers to comparison with the control group. Data are shown as mean ± SD (n = 3). The full-length blots/gels are presented in Supplementary Figs. 8–11

    Article Snippet: HLEC-B3 cells culture and drug treatment The human lens epithelial B3 (HLE-B3) cell line (CRL11421; ATCC, Manassas, Virginia, USA) was used in this experiment.

    Techniques: Fluorescence, Microscopy, Membrane, Expressing, Western Blot, Comparison, Control

    Fig. 7 Apelin-13 regulated the intracellular redox state in H2O2 condi tions. (a) GSH/GSSG of HLE-B3 cells was detected using the GSH/GSSG kit. (b) NADPH/NADP + of HLE-B3 cells was detected using the NADPH/ NADP + kit. Representative images of three replicated experiments were presented. The significance marked at the top of the columns refers to a comparison with the control group. Data are shown as mean ± SD (n = 3)

    Journal: BMC ophthalmology

    Article Title: Protective effect of apelin-13 in lens epithelial cells via inhibiting oxidative stress-induced apoptosis.

    doi: 10.1186/s12886-024-03746-6

    Figure Lengend Snippet: Fig. 7 Apelin-13 regulated the intracellular redox state in H2O2 condi tions. (a) GSH/GSSG of HLE-B3 cells was detected using the GSH/GSSG kit. (b) NADPH/NADP + of HLE-B3 cells was detected using the NADPH/ NADP + kit. Representative images of three replicated experiments were presented. The significance marked at the top of the columns refers to a comparison with the control group. Data are shown as mean ± SD (n = 3)

    Article Snippet: HLEC-B3 cells culture and drug treatment The human lens epithelial B3 (HLE-B3) cell line (CRL11421; ATCC, Manassas, Virginia, USA) was used in this experiment.

    Techniques: Comparison, Control